CATH Classification

Domain Context

CATH Clusters

Superfamily 3.40.50.2300
Functional Family Fructose-like phosphotransferase enzyme IIB component 1

Enzyme Information

2.7.1.202
Protein-N(pi)-phosphohistidine--D-fructose phosphotransferase.
based on mapping to UniProt P69808
[Protein]-N(pi)-phospho-L-histidine + D-fructose(Side 1) = [protein]-L- histidine + D-fructose 1-phosphate(Side 2).
-!- This enzyme is a component (known as enzyme II) of a phosphoenolpyruvate (PEP)-dependent, sugar transporting phosphotransferase system (PTS). -!- The system, which is found only in prokaryotes, simultaneously transports its substrate from the periplasm or extracellular space into the cytoplasm and phosphorylates it. -!- The phosphate donor, which is shared among the different systems, is usually a phospho-carrier protein of low molecular mass that has been phosphorylated by EC 2.7.3.9. -!- The enzyme from the bacterium Escherichia coli is an exception, since it is phosphorylated directly by EC 2.7.3.9. -!- The reaction involves a successive transfer of the phosphate group to several amino acids within the enzyme before the final transfer to the substrate. -!- Formerly EC 2.7.1.69.

UniProtKB Entries (1)

P69808
PTFB1_ECOLI
Escherichia coli K-12
PTS system fructose-like EIIB component 1

PDB Structure

PDB 2KYR
External Links
Method SOLUTION NMR
Organism
Primary Citation
Solution structure of Enzyme IIB subunit of PTS system from Escherichia coli K12, Northeast Structural Genomics Consortium target ER315/Ontario Center for Structural Proteomics target ec0544
Wu, B., Yee, A., Gutmanas, A., Semest, A., Arrowsmith, C.H.
To be Published