CATH Classification

Domain Context

CATH Clusters

Superfamily Hotdog Thioesterase
Functional Family

Enzyme Information

4.2.1.59
3-hydroxyacyl-[acyl-carrier-protein] dehydratase.
based on mapping to UniProt O25928
A (3R)-3-hydroxyacyl-[acyl-carrier protein] = a trans-2-enoyl-[acyl- carrier protein] + H(2)O.
-!- This enzyme is responsible for the dehydration step of the dissociated (type II) fatty-acid biosynthesis system that occurs in plants and bacteria. -!- The enzyme uses fatty acyl thioesters of ACP in vivo. -!- Different forms of the enzyme may have preferences for substrates with different chain length. -!- For example, the activity of FabZ, the ubiquitous enzyme in bacteria, decreases with increasing chain length. -!- Gram-negative bacteria that produce unsaturated fatty acids, such as Escherichia coli, have another form (FabA) that prefers intermediate chain length, and also catalyzes EC 5.3.3.14. -!- Despite the differences both forms can catalyze all steps leading to the synthesis of palmitate (C16:0). -!- FabZ, but not FabA, can also accept unsaturated substrates. -!- Formerly EC 4.2.1.58, EC 4.2.1.60 and EC 4.2.1.61.

UniProtKB Entries (1)

O25928
FABZ_HELPY
Helicobacter pylori 26695
3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ

PDB Structure

PDB 2GLL
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Structural basis for catalytic and inhibitory mechanisms of beta-hydroxyacyl-acyl carrier protein dehydratase (FabZ).
Zhang, L., Liu, W., Hu, T., Du, L., Luo, C., Chen, K., Shen, X., Jiang, H.
J.Biol.Chem.