CATH Classification

Domain Context

CATH Clusters

Superfamily Aldolase class I
Functional Family 2-dehydro-3-deoxy-phosphogluconate/2-dehydro-3-deoxy-6-phosphogalactonate aldolase

Enzyme Information

4.1.2.55
2-dehydro-3-deoxy-phosphogluconate/2-dehydro-3-deoxy-6-phosphogalactonate aldolase.
based on mapping to UniProt O54288
(1) 2-dehydro-3-deoxy-6-phospho-D-gluconate = pyruvate + D-glyceraldehyde 3-phosphate. (2) 2-dehydro-3-deoxy-6-phospho-D-galactonate = pyruvate + D-glyceraldehyde 3-phosphate.
-!- In the archaeon Sulfolobus solfataricus the enzyme is involved in glucose and galactose catabolism via the branched variant of the Entner-Doudoroff pathway. -!- It utilizes 2-dehydro-3-deoxy-6-phosphate-D-gluconate and 2-dehydro- 3-deoxy-6-phosphate-D-galactonate with similar catalytic efficiency. -!- In vitro the enzyme can also catalyze the cleavage of the non- phosphorylated forms 2-dehydro-3-deoxy-D-gluconate and 2-dehydro-3- deoxy-D-galactonate with much lower catalytic efficiency. -!- Cf. EC 4.1.2.21 and EC 4.1.2.14.

UniProtKB Entries (1)

O54288
KDGA_SACSO
Saccharolobus solfataricus
2-dehydro-3-deoxy-phosphogluconate/2-dehydro-3-deoxy-6-phosphogalactonate aldolase

PDB Structure

PDB 1W3I
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
The structural basis for substrate promiscuity in 2-keto-3-deoxygluconate aldolase from the Entner-Doudoroff pathway in Sulfolobus solfataricus.
Theodossis, A., Walden, H., Westwick, E.J., Connaris, H., Lamble, H.J., Hough, D.W., Danson, M.J., Taylor, G.L.
J. Biol. Chem.