CATH Classification

Domain Context

CATH Clusters

Superfamily P-loop containing nucleotide triphosphate hydrolases
Functional Family CTP synthase

Enzyme Information

6.3.4.2
CTP synthase (glutamine hydrolyzing).
based on mapping to UniProt Q5SIA8
ATP + UTP + L-glutamine = ADP + phosphate + CTP + L-glutamate.
-!- The enzyme contains three functionally distinct sites: an allosteric GTP-binding site, a glutaminase site where glutamine hydrolysis occurs (cf. EC 3.5.1.2), and the active site where CTP synthesis takes place. -!- The reaction proceeds via phosphorylation of UTP by ATP to give an activated intermediate 4-phosphoryl UTP and ADP. -!- Ammonia then reacts with this intermediate generating CTP and a phosphate. -!- The enzyme can also use ammonia from the surrounding solution.

UniProtKB Entries (1)

Q5SIA8
PYRG_THET8
Thermus thermophilus HB8
CTP synthase

PDB Structure

PDB 1VCO
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Crystal Structures of CTP Synthetase Reveal ATP, UTP, and Glutamine Binding Sites
Goto, M., Omi, R., Nakagawa, N., Miyahara, I., Hirotsu, K.
Structure