CATH Classification

Domain Context

CATH Clusters

Superfamily 3.40.50.2300
Functional Family Galactitol-specific phosphotransferase enzyme IIB component

Enzyme Information

2.7.1.200
Protein-N(pi)-phosphohistidine--galactitol phosphotransferase.
based on mapping to UniProt P0A435
[Protein]-N(pi)-phospho-L-histidine + galactitol(Side 1) = [protein]-L- histidine + galactitol 1-phosphate(Side 2).
-!- This enzyme is a component (known as enzyme II) of a phosphoenolpyruvate (PEP)-dependent, sugar transporting phosphotransferase system (PTS). -!- The system, which is found only in prokaryotes, simultaneously transports its substrate from the periplasm or extracellular space into the cytoplasm and phosphorylates it. -!- The phosphate donor, which is shared among the different systems, is a phospho-carrier protein of low molecular mass that has been phosphorylated by EC 2.7.3.9. -!- Enzyme II, on the other hand, is specific for a particular substrate, although in some cases alternative substrates can be transported with lower efficiency. -!- The reaction involves a successive transfer of the phosphate group to several amino acids within the enzyme before the final transfer to the substrate. -!- Formerly EC 2.7.1.69.

UniProtKB Entries (1)

P0A435
PTKB_ECOL6
Escherichia coli CFT073
PTS system galactitol-specific EIIB component

PDB Structure

PDB 1TVM
External Links
Method SOLUTION NMR
Organism Escherichia
Primary Citation
NMR structure of the enzyme GatB of the galactitol-specific phosphoenolpyruvate-dependent phosphotransferase system and its interaction with GatA.
Volpon, L., Young, C.R., Matte, A., Gehring, K.
Protein Sci.