CATH Domain 5cq2A02

CATH Classification

Level	CATH Code	Description
	2	Mainly Beta
	2.20	Single Sheet
	2.20.70	Ubiquitin Ligase Nedd4; Chain: W;
	2.20.70.10

Domain Context

CATH Clusters

Superfamily	2.20.70.10
Functional Family	E3 ubiquitin-protein ligase NEDD4

Enzyme Information

2.3.2.26

HECT-type E3 ubiquitin transferase.

based on mapping to UniProt Q96J02

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)- ubiquitinyl-[acceptor protein]-L-lysine.

-!- In the first step the enzyme transfers ubiquitin from the E2 ubiquitin-conjugating enzyme (EC 2.3.2.23) to a cysteine residue in its HECT domain (which is located in the C-terminal region), forming a thioester bond. -!- In a subsequent step the enzyme transfers the ubiquitin to an acceptor protein, resulting in the formation of an isopeptide bond between the C-terminal glycine residue of ubiquitin and the epsilon- amino group of an L-lysine residue of the acceptor protein. -!- Cf. EC 2.3.2.27 and EC 2.3.2.31.

UniProtKB Entries (1)

Q9H3M7

TXNIP_HUMAN

Homo sapiens

Thioredoxin-interacting protein

PDB Structure

PDB	5CQ2
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	Structural basis for the regulatory role of the PPxY motifs in the thioredoxin-interacting protein TXNIP. Liu, Y., Lau, J., Li, W., Tempel, W., Li, L., Dong, A., Narula, A., Qin, S., Min, J. Biochem.J.

Network disruptions