CATH Domain 3nosA02

CATH Classification

Level	CATH Code	Description
	3	Alpha Beta
	3.90	Alpha-Beta Complex
	3.90.440	Nitric Oxide Synthase;Heme Domain; Chain A, domain 2
	3.90.440.10	Nitric Oxide Synthase;Heme Domain;Chain A domain 2

Domain Context

CATH Clusters

Superfamily	Nitric Oxide Synthase;Heme Domain;Chain A domain 2
Functional Family	Endothelial nitric oxide synthase

Enzyme Information

1.14.13.39

Nitric-oxide synthase (NADPH).

based on mapping to UniProt P29474

2 L-arginine + 3 NADPH + 4 O(2) = 2 L-citrulline + 2 nitric oxide + 3 NADP(+) + 4 H(2)O.

-!- The enzyme consists of linked oxygenase and reductase domains. -!- The eukaryotic enzyme binds FAD, FMN, heme (iron protoporphyrin IX) and tetrahydrobiopterin, and its two domains are linked via a regulatory calmodulin-binding domain. -!- Upon calcium-induced calmodulin binding, the reductase and oxygenase domains form a complex, allowing electrons to flow from NADPH via FAD and FMN to the active center. -!- The reductase domain of the enzyme from the bacterium Sorangium cellulosum utilizes a [2Fe-2S] cluster to transfer the electrons from NADPH to the active center. -!- Cf. EC 1.14.14.47.

UniProtKB Entries (1)

P29474

NOS3_HUMAN

Homo sapiens

Nitric oxide synthase, endothelial

PDB Structure

PDB	3NOS
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism	Escherichia
Primary Citation	Structural characterization of nitric oxide synthase isoforms reveals striking active-site conservation. Fischmann, T.O., Hruza, A., Niu, X.D., Fossetta, J.D., Lunn, C.A., Dolphin, E., Prongay, A.J., Reichert, P., Lundell, D.J., Narula, S.K., Weber, P.C. Nat.Struct.Biol.