CATH Domain 2jmfA01

CATH Classification

Level	CATH Code	Description
	2	Mainly Beta
	2.20	Single Sheet
	2.20.70	Ubiquitin Ligase Nedd4; Chain: W;
	2.20.70.10

Domain Context

CATH Clusters

Superfamily	2.20.70.10
Functional Family

Enzyme Information

2.3.2.26

HECT-type E3 ubiquitin transferase.

based on mapping to UniProt Q9Y0H4

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)- ubiquitinyl-[acceptor protein]-L-lysine.

-!- In the first step the enzyme transfers ubiquitin from the E2 ubiquitin-conjugating enzyme (EC 2.3.2.23) to a cysteine residue in its HECT domain (which is located in the C-terminal region), forming a thioester bond. -!- In a subsequent step the enzyme transfers the ubiquitin to an acceptor protein, resulting in the formation of an isopeptide bond between the C-terminal glycine residue of ubiquitin and the epsilon- amino group of an L-lysine residue of the acceptor protein. -!- Cf. EC 2.3.2.27 and EC 2.3.2.31.

UniProtKB Entries (1)

Q32UW5

Q32UW5_DROME

Drosophila melanogaster

Notch

PDB Structure

PDB	2JMF
External Links	PDBSum Proteopedia
Method	SOLUTION NMR
Organism	Escherichia
Primary Citation	Specificity and Autoregulation of Notch Binding by Tandem WW Domains in Suppressor of Deltex Jennings, M.D., Blankley, R.T., Baron, M., Golovanov, A.P., Avis, J.M. J.Biol.Chem.

Network disruptions