CATH Classification
| Level | CATH Code | Description |
|---|---|---|
|
3 | Alpha Beta |
|
3.40 | 3-Layer(aba) Sandwich |
|
3.40.50 | Rossmann fold |
|
3.40.50.1820 | alpha/beta hydrolase |
Domain Context
CATH Clusters
| Superfamily | alpha/beta hydrolase |
| Functional Family | Narbonolide/10-deoxymethynolide synthase PikA4, module 6 |
Enzyme Information
| 2.3.1.240 |
Narbonolide synthase.
based on mapping to UniProt Q9ZGI2
Malonyl-CoA + 6 (2S)-methylmalonyl-CoA + 5 NADPH = narbonolide + 7 CoA + 7 CO(2) + 5 NADP(+) + 2 H(2)O.
-!- The product, narbonolide, contains a 14-membered ring and is an intermediate in the biosynthesis of narbonomycin and pikromycin in the bacterium Streptomyces venezuelae. -!- The enzyme also produces 10-deoxymethynolide (see EC 2.3.1.239). -!- The enzyme has 29 active sites arranged in four polypeptides (pikAI - pikAIV) with a loading domain, six extension modules and a terminal thioesterase domain. -!- Each extension module contains a ketosynthase (KS), keto reductase (KR), an acyltransferase (AT) and an acyl-carrier protein (ACP). -!- Not all active sites are used in the biosynthesis.
|
| 2.3.1.239 |
10-deoxymethynolide syntase.
based on mapping to UniProt Q9ZGI2
Malonyl-CoA + 5 (2S)-methylmalonyl-CoA + 5 NADPH = 10-deoxymethynolide + 6 CoA + 6 CO(2) + 5 NADP(+) + 2 H(2)O.
-!- The product, 10-deoxymethynolide, contains a 12-membered ring and is an intermediate in the biosynthesis of methymycin in the bacterium Streptomyces venezuelae. -!- The enzyme also produces narbonolide (see EC 2.3.1.240). -!- The enzyme has 29 active sites arranged in four polypeptides (pikAI - pikAIV) with a loading domain, six extension modules and a terminal thioesterase domain. -!- Each extension module contains a ketosynthase (KS), keto reductase (KR), an acyltransferase (AT) and an acyl-carrier protein (ACP). -!- Not all active sites are used in the biosynthesis.
|
UniProtKB Entries (1)
| Q9ZGI2 |
PIKA4_STRVZ
Streptomyces venezuelae
Narbonolide/10-deoxymethynolide synthase PikA4, module 6
|
PDB Structure
| PDB | 2HFJ |
| External Links | |
| Method | X-RAY DIFFRACTION |
| Organism | Escherichia |
| Primary Citation |
Structural Basis for Macrolactonization by the Pikromycin Thioesterase
NAT.CHEM.BIOL.
|
