CATH Domain 2chnB04

CATH Classification

Level	CATH Code	Description
	2	Mainly Beta
	2.60	Sandwich
	2.60.40	Immunoglobulin-like
	2.60.40.1180	Golgi alpha-mannosidase II

Domain Context

CATH Clusters

Superfamily	Golgi alpha-mannosidase II
Functional Family

Enzyme Information

3.2.1.169

Protein O-GlcNAcase.

based on mapping to UniProt Q89ZI2

(1) [Protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H(2)O = [protein]-L-serine + N-acetyl-D-glucosamine. (2) [Protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine + H(2)O = [protein]-L-threonine + N-acetyl-D-glucosamine.

-!- Within higher eukaryotes post-translational modification of protein serines/threonines with N-acetylglucosamine (O-GlcNAc) is dynamic, inducible and abundant, regulating many cellular processes by interfering with protein phosphorylation. -!- EC 2.4.1.155 transfers GlcNAc onto substrate proteins and EC 3.2.1.169 cleaves GlcNAc from the modified proteins.

UniProtKB Entries (1)

Q89ZI2

OGA_BACTN

Bacteroides thetaiotaomicron VPI-5482

O-GlcNAcase BT_4395

PDB Structure

PDB	2CHN
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	Structure and Mechanism of a Bacterial B-Glucosaminidase Having O-Glcnacase Activity Dennis, R.J., Taylor, E.J., Macauley, M.S., Stubbs, K.A., Turkenburg, J.P., Hart, S.J., Black, G.N., Vocadlo, D.J., Davies, G.J. Nat.Struct.Mol.Biol.

Network disruptions