The name of this superfamily has been modified since the most recent official CATH+ release (v4_3_0). At the point of the last release, this superfamily was named:

Mediator complex subunit 25, ACID domain

Functional Families

Overview of the Structural Clusters (SC) and Functional Families within this CATH Superfamily. Clusters with a representative structure are represented by a filled circle.

Superfamily: Mediator complex subunit 25, ACID domain

Mediator is a large complex of up to 33 proteins that is conserved from plants to fungi to humans - the number and representation of individual subunits varying with species PMID:14983011,PMID:17560376. It is arranged into four different sections, a core, a head, a tail and a kinase-active part, and the number of subunits within each of these is what varies with species. Overall, Mediator regulates the transcriptional activity of RNA polymerase II, but it would appear that each of the four different sections has a slightly different function PMID:18381891. The overall function of the full-length Med25 is efficiently to coordinate the transcriptional activation of RAR/RXR (retinoic acid receptor/retinoic X receptor) in higher eukaryotic cells. Human Med25 consists of several domains with different binding properties, the N-terminal, VWA domain, an SD1 - synapsin 1 - domain from residues 229-381, a PTOV(B) or ACID domain from 395-545, an SD2 domain from residues 564-645 and a C-terminal NR box-containing domain (646-650) from 646-747.

This superfamily includes the ACID domain (activator-interacting domain). Unlike other known activator targets, ACID forms a seven-stranded beta-barrel framed by three helices. The ACID domain appears to impose a critical control on transcription activation by VP16 in mammalian cells, uncovering a novel molecular activation pathway of VP16 through transcriptional complexes containing the MED25 subunit.

INTERPRO:IPR021394, PFAM:PF11232,DOI:10.1007/s10969-011-9115-1,DOI:10.1016/j.jsb.2010.10.011,DOI:10.1038/nsmb.1999,DOI:10.1038/nsmb.1997

GO Diversity

Unique GO annotations
20 Unique GO terms

EC Diversity

Unique EC annotations
0 Unique EC terms

Species Diversity

Unique species annotations
256 Unique species

Sequence/Structure Diversity

Overview of the sequence / structure diversity of this superfamily compared to other superfamilies in CATH. Click on the chart to view the data in more detail.

Superfamily Summary

A general summary of information for this superfamily.
Domains: 4
Domain clusters (>95% seq id): 3
Domain clusters (>35% seq id): 1
Unique PDBs: 4
Structural Clusters (5A): 1
Structural Clusters (9A): 1
FunFam Clusters: 11
Unique EC:
Unique GO: 20
Unique Species: 256