CATH Classification

Domain Context

CATH Clusters

Superfamily Cysteine proteinases
Functional Family Ubiquitin carboxyl-terminal hydrolase 8

Enzyme Information

3.4.19.12
Ubiquitinyl hydrolase 1.
based on mapping to UniProt P50102
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
-!- Links to polypeptides smaller than 60 residues are hydrolyzed more readily than those to larger polypeptides. -!- Isoforms exist with quantitatively different specificities among the best known being UCH-L1 and UCH-L3, major proteins of the brain of mammals. -!- Inhibited by ubiquitin aldehyde (in which Gly76 is replaced by aminoacetaldehyde). -!- Belongs to peptidase family C12.

UniProtKB Entries (2)

P0CG48
UBC_HUMAN
Homo sapiens
Polyubiquitin-C
Q6WNK7
SUS1_YEAST
Saccharomyces cerevisiae S288C
Transcription and mRNA export factor SUS1

PDB Structure

PDB 6AQR
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Active site alanine mutations convert deubiquitinases into high-affinity ubiquitin-binding proteins.
Morrow, M.E., Morgan, M.T., Clerici, M., Growkova, K., Yan, M., Komander, D., Sixma, T.K., Simicek, M., Wolberger, C.
EMBO Rep.