CATH Classification

Domain Context

CATH Clusters

Superfamily 3.40.50.970
Functional Family

Enzyme Information

2.2.1.9
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase.
based on mapping to UniProt P23970
Isochorismate + 2-oxoglutarate = 5-enolpyruvoyl-6-hydroxy-2-succinyl- cyclohex-3-ene-1-carboxylate + CO(2).
-!- Involved in the biosynthesis of vitamin K(2) (menaquinone). -!- In most anaerobes and all Gram-positive aerobes, menaquinone is the sole electron transporter in the respiratory chain and is essential for their survival. -!- It had previously been thought that the products of the reaction were (1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate (SHCHC), pyruvate and CO(2) but it is now known that two separate enzymes are involved: this enzyme and EC 4.2.99.20. -!- Under basic conditions, the product can spontaneously lose pyruvate to form SHCHC. -!- Formerly EC 2.5.1.64 and EC 2.5.1.n1.

UniProtKB Entries (1)

P23970
MEND_BACSU
Bacillus subtilis subsp. subtilis str. 168
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase

PDB Structure

PDB 2X7J
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Structure and Reactivity of Bacillus Subtilis Mend Catalyzing the First Committed Step in Menaquinone Biosynthesis.
Dawson, A., Chen, M., Fyfe, P.K., Guo, Z., Hunter, W.N.
J.Mol.Biol.