CATH Domain 2nq3A00

CATH Classification

Level	CATH Code	Description
	2	Mainly Beta
	2.60	Sandwich
	2.60.40	Immunoglobulin-like
	2.60.40.150	C2 domain

Domain Context

CATH Clusters

Superfamily	C2 domain
Functional Family	E3 ubiquitin-protein ligase

Enzyme Information

2.3.2.26

HECT-type E3 ubiquitin transferase.

based on mapping to UniProt Q96J02

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)- ubiquitinyl-[acceptor protein]-L-lysine.

-!- In the first step the enzyme transfers ubiquitin from the E2 ubiquitin-conjugating enzyme (EC 2.3.2.23) to a cysteine residue in its HECT domain (which is located in the C-terminal region), forming a thioester bond. -!- In a subsequent step the enzyme transfers the ubiquitin to an acceptor protein, resulting in the formation of an isopeptide bond between the C-terminal glycine residue of ubiquitin and the epsilon- amino group of an L-lysine residue of the acceptor protein. -!- Cf. EC 2.3.2.27 and EC 2.3.2.31.

UniProtKB Entries (1)

Q96J02

ITCH_HUMAN

Homo sapiens

E3 ubiquitin-protein ligase Itchy homolog

PDB Structure

PDB	2NQ3
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism	Escherichia
Primary Citation	The C2 Domain of Human Itchy Homolog E3 Ubiquitin Protein Ligase Walker, J.R., Avvakumov, G.V., Xue, S., Butler-Cole, C., Finerty Jr., P.J., Weigelt, J., Sundstrom, M., Arrowsmith, C.H., Edwards, A.M., Bochkarev, A., Dhe-Paganon, S. To be Published

Network disruptions