CATH Classification

Domain Context

CATH Clusters

Superfamily Single-stranded right-handed beta-helix, Pectin lyase-like
Functional Family

Enzyme Information
Chondroitin B lyase.
based on mapping to UniProt Q46079
Eliminative cleavage of dermatan sulfate containing (1->4)-beta-D- hexosaminyl and (1->3)-beta-D-glucurosonyl or (1->3)-alpha-L-iduronosyl linkages to disaccharides containing 4-deoxy-beta-D-gluc-4-enuronosyl groups to yield a 4,5-unsaturated dermatan-sulfate disaccharide (Delta- UA-GalNAc-4S).
-!- This is the only lyase that is known to be specific for dermatan sulfate as substrate. -!- The minimum substrate length required for catalysis is a tetrasaccharide. -!- In general, chondroitin sulfate (CS) and dermatan sulfate (DS) chains comprise a linkage region, a chain cap and a repeat region. -!- The repeat region of CS is a repeating disaccharide of glucuronic acid (GlcA) and N-acetylgalactosamine (GalNAc) [-4)GlcA(beta-1- 3)GalNAc(beta-1-](n), which may be O-sulfated on the C-4 and/or C-6 of GalNAc and C-2 of GlcA. -!- GlcA residues of CS may be epimerized to iduronic acid (IdoA) forming the repeating disaccharide [-4)IdoA(alpha-1-3)GalNAc(beta-1-](n) of DS. -!- Both the concentrations and locations of sulfate-ester substituents vary with glucosaminoglycan source.

UniProtKB Entries (1)

Pedobacter heparinus DSM 2366

PDB Structure

External Links
Primary Citation
The Structure of Chondroitin B Lyase Complexed with Glycosaminoglycan Oligosaccharides Unravels a Calcium-Dependent Catalytic Machinery
Michel, G., Pojasek, K., Li, Y., Sulea, T., Linhardt, R., Raman, R., Prabhakar, V., Sasisekharan, R., Cygler, M.