CATH Classification

Domain Context

CATH Clusters

Superfamily Nitric Oxide Synthase; Chain A, domain 3
Functional Family Nitric oxide synthase, brain

Enzyme Information
Nitric-oxide synthase (NADPH).
based on mapping to UniProt P29476
2 L-arginine + 3 NADPH + 4 O(2) = 2 L-citrulline + 2 nitric oxide + 3 NADP(+) + 4 H(2)O.
-!- The enzyme consists of linked oxygenase and reductase domains. -!- The eukaryotic enzyme binds FAD, FMN, heme (iron protoporphyrin IX) and tetrahydrobiopterin, and its two domains are linked via a regulatory calmodulin-binding domain. -!- Upon calcium-induced calmodulin binding, the reductase and oxygenase domains form a complex, allowing electrons to flow from NADPH via FAD and FMN to the active center. -!- The reductase domain of the enzyme from the bacterium Sorangium cellulosum utilizes a [2Fe-2S] cluster to transfer the electrons from NADPH to the active center. -!- Cf. EC

UniProtKB Entries (1)

Rattus norvegicus
Nitric oxide synthase, brain

PDB Structure

External Links
Organism Escherichia
Primary Citation
Structure of rat brain nNOS heme domain
Li, H., Martasek, P., Masters, B.S.S., Poulos, T.L., Raman, C.S.
To be Published