CATH Classification

Domain Context

CATH Clusters

Superfamily 2.60.120.200
Functional Family Exotoxin A

Enzyme Information

2.4.2.36
NAD(+)--diphthamide ADP-ribosyltransferase.
based on mapping to UniProt P11439
NAD(+) + diphthamide-[translation elongation factor 2] = nicotinamide + N-(ADP-D-ribosyl)diphthamide-[translation elongation factor 2].
-!- Dihtheria toxin and some other bacterial toxins catalyze this reaction, which inactivates translation elongation factor 2 (EF2). -!- The acceptor is diphthamide, a unique modification of a histidine residue in the elongation factor found in archaebacteria and all eukaryotes, but not in eubacteria. -!- Cf. EC 2.4.2.31. -!- The relevant histidine of EF2 is His(715) in mammals, His(699) in yeast and His(600) in Pyrococcus horikoshii.

UniProtKB Entries (1)

P11439
TOXA_PSEAE
Pseudomonas aeruginosa PAO1
Exotoxin A

PDB Structure

PDB 1IKQ
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Refined Crystallographic Structure of Pseudomonas aeruginosa Exotoxin A and its Implications for the Molecular Mechanism of Toxicity
Wedekind, J.E., Trame, C.B., Dorywalska, M., Koehl, P., Raschke, T.M., McKee, M., FitzGerald, D., Collier, R.J., McKay, D.B.
J.Mol.Biol.