CATH Classification
| Level | CATH Code | Description |
|---|---|---|
|
2 | Mainly Beta |
|
2.140 | 8 Propeller |
|
2.140.10 | Methanol Dehydrogenase; Chain A |
|
2.140.10.10 | Quinoprotein alcohol dehydrogenase-like superfamily |
Domain Context
CATH Clusters
| Superfamily | Quinoprotein alcohol dehydrogenase-like superfamily |
| Functional Family | Methanol dehydrogenase, large subunit |
Enzyme Information
| 1.1.2.8 |
Alcohol dehydrogenase (cytochrome c).
based on mapping to UniProt Q9Z4J7
A primary alcohol + 2 cytochrome c = an aldehyde + 2 reduced cytochrome c + 2 H(+).
-!- A periplasmic PQQ-containing quinoprotein. -!- Occurs in Pseudomonas and Rhodopseudomonas. -!- The enzyme from Pseudomonas aeruginosa uses a specific inducible cytochrome c(550) as electron acceptor. -!- Acts on a wide range of primary and secondary alcohols, but not methanol. -!- It has a homodimeric structure (contrasting with the heterotetrameric structure of EC 1.1.2.7, methanol dehydrogenase (cytochrome c)). -!- It is routinely assayed with phenazine methosulfate as electron acceptor. -!- Activity is stimulated by ammonia or amines. -!- Like all other quinoprotein alcohol dehydrogenases it has an 8-bladed 'propeller' structure, a calcium ion bound to the PQQ in the active site and an unusual disulfide ring structure in close proximity to the PQQ. -!- Formerly EC 1.1.99.8.
|
UniProtKB Entries (1)
| Q9Z4J7 |
EXAA_PSEAE
Pseudomonas aeruginosa PAO1
Quinoprotein alcohol dehydrogenase (cytochrome c)
|
PDB Structure
| PDB | 1FLG |
| External Links | |
| Method | X-RAY DIFFRACTION |
| Organism | |
| Primary Citation |
X-ray structure of the quinoprotein ethanol dehydrogenase from Pseudomonas aeruginosa: basis of substrate specificity.
J.Mol.Biol.
|
