CATH Classification

Domain Context

CATH Clusters

Superfamily Nitric Oxide Synthase; Chain A, domain 3
Functional Family Nitric oxide synthase, brain

Enzyme Information
Nitric-oxide synthase (NADPH).
based on mapping to UniProt P29473
2 L-arginine + 3 NADPH + 4 O(2) = 2 L-citrulline + 2 nitric oxide + 3 NADP(+) + 4 H(2)O.
-!- The enzyme consists of linked oxygenase and reductase domains. -!- The eukaryotic enzyme binds FAD, FMN, heme (iron protoporphyrin IX) and tetrahydrobiopterin, and its two domains are linked via a regulatory calmodulin-binding domain. -!- Upon calcium-induced calmodulin binding, the reductase and oxygenase domains form a complex, allowing electrons to flow from NADPH via FAD and FMN to the active center. -!- The reductase domain of the enzyme from the bacterium Sorangium cellulosum utilizes a [2Fe-2S] cluster to transfer the electrons from NADPH to the active center. -!- Cf. EC

UniProtKB Entries (1)

Bos taurus
Nitric oxide synthase, endothelial

PDB Structure

External Links
Organism Escherichia
Primary Citation
Implications for isoform-selective inhibitor design derived from the binding mode of bulky isothioureas to the heme domain of endothelial nitric-oxide synthase.
Raman, C.S., Li, H., Martasek, P., Babu, B.R., Griffith, O.W., Masters, B.S., Poulos, T.L.