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1 keywords

CATH Domain 3tykA02

CATH Classification

Level CATH Code Description
Class 3 Alpha Beta
Architecture 3.90 Alpha-Beta Complex
Topology 3.90.1200 Aminoglycoside 3'-phosphotransferase; Chain: A, domain 2
Homologous Superfamily 3.90.1200.10 Aminoglycoside phosphotransferase (APH), C-terminal lobe

Domain Context

CATH Clusters

Superfamily 3.90.1200.10
Functional Family

Enzyme Information

2.7.1.163
Hygromycin B 4-O-kinase.
based on mapping to UniProt P00557
ATP + hygromycin B = ADP + 4-O-phosphohygromycin B.
-!- Phosphorylates the antibiotic hygromycin B. -!- Whereas the enzyme from Streptomyces hygroscopicus (EC 2.7.1.119) catalyzes the formation of 7''-O-phosphohygromycin B, this enzyme, found in Escherichia coli carrying a plasmid conferring resistance to hygromycin-B, forms 4-O-phosphohygromycin B. -!- Formerly EC 2.7.1.n6.

UniProtKB Entries (1)

P00557
KHYB_ECOLX
Escherichia coli
Hygromycin-B 4-O-kinase

PDB Structure

PDB 3TYK
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Structure and function of APH(4)-Ia, a hygromycin B resistance enzyme.
Stogios, P.J., Shakya, T., Evdokimova, E., Savchenko, A., Wright, G.D.
J.Biol.Chem.
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