CATH Classification

Domain Context

CATH Clusters

Superfamily Ubiquitin Conjugating Enzyme
Functional Family Ubiquitin-conjugating enzyme E2-21 kDa

Enzyme Information

2.3.2.23
E2 ubiquitin-conjugating enzyme.
based on mapping to UniProt P29340
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L- cysteine.
-!- The E2 ubiquitin-conjugating enzyme acquires the activated ubquitin from the E1 ubiquitin-activating enzyme (EC 6.2.1.45) and binds it via a transthioesterification reaction to itself. -!- In the human enzyme the catalytic center is located at Cys-87 where ubiquitin is bound via its C-terminal glycine in a thioester linkage. -!- Formerly EC 6.3.2.19.

UniProtKB Entries (1)

P39718
PEX22_YEAST
Saccharomyces cerevisiae S288C
Peroxisome assembly protein 22

PDB Structure

PDB 4BWF
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
A Disulphide Bond in the E2 Enzyme Pex4P Modulates Ubiquitin-Conjugating Activity
Williams, C., van den Berg, M., Stanley, W.A., Wilmanns, M., Distel, B.
Sci.Rep.