CATH Classification

Domain Context

CATH Clusters

Superfamily 3.40.630.30
Functional Family Glycylpeptide N-tetradecanoyltransferase

Enzyme Information

2.3.1.97
Glycylpeptide N-tetradecanoyltransferase.
based on mapping to UniProt P14743
Tetradecanoyl-CoA + an N-terminal-glycyl-[protein] = CoA + an N-terminal- N-tetradecanoylglycyl-[protein].
-!- The enzyme catalyzes the transfer of myristic acid from myristoyl-CoA to the amino group of the N-terminal glycine residue in a variety of eukaryotic proteins. -!- It uses an ordered Bi Bi reaction in which myristoyl-CoA binds to the enzyme prior to the binding of the peptide substrate, and CoA release precedes the release of the myristoylated peptide. -!- The enzyme from yeast is profoundly affected by amino acids further from the N-terminus, and is particularly stimulated by a serine residue at position 5.

UniProtKB Entries (1)

P14743
NMT_YEAST
Saccharomyces cerevisiae S288C
Glycylpeptide N-tetradecanoyltransferase

PDB Structure

PDB 2P6G
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Crystal structures of Saccharomyces cerevisiae N-myristoyltransferase with bound myristoyl-CoA and inhibitors reveal the functional roles of the N-terminal region.
Wu, J., Tao, Y., Zhang, M., Howard, M.H., Gutteridge, S., Ding, J.
J.Biol.Chem.