CATH Classification

Domain Context

CATH Clusters

Superfamily DNA-directed RNA polymerase, insert domain
Functional Family DNA-directed RNA polymerase subunit alpha

Enzyme Information

2.7.7.6
DNA-directed RNA polymerase.
based on mapping to UniProt P0A7Z4
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
-!- Catalyzes DNA-template-directed extension of the 3'-end of an RNA strand by one nucleotide at a time. -!- Can initiate a chain de novo. -!- In eukaryotes three forms of the enzyme have been distinguished on the basis of sensitivity of alpha-amanitin, and the type of RNA synthesized. -!- See also EC 2.7.7.19 and EC 2.7.7.48.

UniProtKB Entries (1)

P00579
RPOD_ECOLI
Escherichia coli K-12
RNA polymerase sigma factor RpoD

PDB Structure

PDB 6BYU
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Structure-function comparisons of (p)ppApp vs (p)ppGpp for Escherichia coli RNA polymerase binding sites and for rrnB P1 promoter regulatory responses in vitro.
Bruhn-Olszewska, B., Molodtsov, V., Sobala, M., Dylewski, M., Murakami, K.S., Cashel, M., Potrykus, K.
Biochim. Biophys. Acta