CATH Classification

Domain Context

CATH Clusters

Superfamily DNA-directed RNA polymerase, insert domain
Functional Family DNA-directed RNA polymerase subunit alpha

Enzyme Information

2.7.7.6
DNA-directed RNA polymerase.
based on mapping to UniProt Q72I32
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
-!- Catalyzes DNA-template-directed extension of the 3'-end of an RNA strand by one nucleotide at a time. -!- Can initiate a chain de novo. -!- In eukaryotes three forms of the enzyme have been distinguished on the basis of sensitivity of alpha-amanitin, and the type of RNA synthesized. -!- See also EC 2.7.7.19 and EC 2.7.7.48.

UniProtKB Entries (2)

Q8RQE7
RPOZ_THET8
Thermus thermophilus HB8
DNA-directed RNA polymerase subunit omega
Q72I32
RPOA_THET2
Thermus thermophilus HB27
DNA-directed RNA polymerase subunit alpha

PDB Structure

PDB 5VOI
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
X-ray crystal structure of a reiterative transcription complex reveals an atypical RNA extension pathway.
Murakami, K.S., Shin, Y., Turnbough, C.L., Molodtsov, V.
Proc. Natl. Acad. Sci. U.S.A.