CATH Classification

Domain Context

CATH Clusters

Superfamily Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)
Functional Family Adenine DNA glycosylase

Enzyme Information

3.2.2.31
Adenine glycosylase.
based on mapping to UniProt P83847
Hydrolyzes free adenine bases from 7,8-dihydro-8-oxoguanine:adenine mismatched double-stranded DNA, leaving an apurinic site.
-!- The enzyme serves as a mismatch repair enzyme that works to correct 7,8-dihydro-8-oxoguanine:adenine mispairs that arise in DNA when error-prone synthesis occurs past 7,8-dihydro-8-oxoguanine (GO) lesions in DNA. -!- The enzyme excises the adenine of the mispair, producing an apurinic site sensitive to AP endonuclease activity. -!- After removing the undamaged adenine the enzyme remains bound to the site to prevent EC 3.2.2.23 (MutM) from removing the GO lesion, which could lead to a double strand break. -!- In vitro the enzyme is also active with adenine:guanine, adenine:cytosine, and adenine:7,8-dihydro-8-oxoadenine (AO) mispairs, removing the adenine in all cases.

UniProtKB Entries (1)

P83847
MUTY_GEOSE
Geobacillus stearothermophilus
Adenine DNA glycosylase

PDB Structure

PDB 5KN8
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Structural Basis for the Lesion-scanning Mechanism of the MutY DNA Glycosylase.
Wang, L., Chakravarthy, S., Verdine, G.L.
J. Biol. Chem.