CATH Classification

Domain Context

CATH Clusters

Superfamily Flavivirus RNA-directed RNA polymerase, thumb domain
Functional Family

Enzyme Information

3.4.21.91
Flavivirin.
based on mapping to UniProt Q6YMS4
Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.
2.1.1.57
Methyltransferase cap1.
based on mapping to UniProt Q6YMS4
S-adenosyl-L-methionine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)- (purine-ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)- methyl 5'-triphosphoguanosine)-(2'-O-methyl-purine-ribonucleotide)- [mRNA].
-!- This enzyme catalyzes the methylation of the ribose on the first transcribed nucleotide of mRNA or snRNA molecules, which may be either guanosine or adenosine. -!- This methylation event is known as cap1, and occurs in all mRNAs and snRNAs of higher eukaryotes, including insects, vertebrates and their viruses. -!- The human enzyme can also methylate mRNA molecules that lack methylation on the capping 5'-triphosphoguanosine. -!- Formerly EC 2.1.1.58.
2.7.7.48
RNA-directed RNA polymerase.
based on mapping to UniProt Q6YMS4
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
-!- Catalyzes RNA-template-directed extension of the 3'-end of an RNA strand by one nucleotide at a time. -!- Can initiate a chain de novo. -!- See also EC 2.7.7.6.
3.6.1.15
Nucleoside-triphosphate phosphatase.
based on mapping to UniProt Q6YMS4
NTP + H(2)O = NDP + phosphate.
-!- The enzyme is found in eukaryotes and thermophilic bacteria, but appears to be absent from mesophilic bacteria. -!- Also hydrolyzes nucleoside diphosphates, thiamine diphosphate and FAD. -!- The enzyme from the plant Pisum sativum (garden pea) is regulated by calmodulin.
3.6.4.13
RNA helicase.
based on mapping to UniProt Q6YMS4
ATP + H(2)O = ADP + phosphate.
-!- RNA helicases utilize the energy from ATP hydrolysis to unwind RNA. -!- Some of them unwind RNA with a 3' to 5' polarity, other show 5' to 3' polarity. -!- Some helicases unwind DNA as well as RNA. -!- May be identical with EC 3.6.4.12 (DNA helicase).
2.1.1.56
mRNA (guanine-N(7)-)-methyltransferase.
based on mapping to UniProt Q6YMS4
S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m(7)G(5')pppR-RNA.
-!- Adds an N(7)-methylguanine cap to mRNA. -!- The nucleoside next to the terminal guanosine may be either guanosine or adenosine.

UniProtKB Entries (1)

Q6YMS4
POLG_DEN3S
Dengue virus 3 Sri Lanka/1266/2000
Genome polyprotein

PDB Structure

PDB 5I3Q
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Potent Allosteric Dengue Virus NS5 Polymerase Inhibitors: Mechanism of Action and Resistance Profiling
Lim, S.P., Noble, C.G., Seh, C.C., Soh, T.S., El Sahili, A., Chan, G.K., Lescar, J., Arora, R., Benson, T., Nilar, S., Manjunatha, U., Wan, K.F., Dong, H., Xie, X., Shi, P.Y., Yokokawa, F.
Plos Pathog.