CATH Classification

Domain Context

CATH Clusters

Superfamily Nucleic acid-binding proteins
Functional Family

Enzyme Information

6.1.1.23
Aspartate--tRNA(Asn) ligase.
based on mapping to UniProt P56459
ATP + L-aspartate + tRNA(Asx) = AMP + diphosphate + L-aspartyl-tRNA(Asx).
-!- When this enzyme acts on tRNA(Asp), it catalyzes the same reaction as EC 6.1.1.12. -!- It has, however, diminished discrimination, so that it can also form aspartyl-tRNA(Asn). -!- This relaxation of specificity has been found to result from the absence of a loop in the tRNA that specifically recognizes the third position of the anticodon. -!- This accounts for the ability of this enzyme in, for example, Thermus thermophilus, to recognize both tRNA(Asp) (GUC anticodon) and tRNA(Asn) (GUU anticodon). -!- The aspartate-tRNA(Asn) is not used in protein synthesis until it is converted by EC 6.3.5.6 into asparaginyl-tRNA(Asn).

UniProtKB Entries (1)

P56459
SYDND_HELPY
Helicobacter pylori 26695
Aspartate--tRNA(Asp/Asn) ligase

PDB Structure

PDB 5GRO
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Crystal structure of the N-terminal anticodon-binding domain of the nondiscriminating aspartyl-tRNA synthetase from Helicobacter pylori
Songsiriritthigul, C., Suebka, S., Chen, C.-J., Fuengfuloy, P., Chuawong, P.
Acta Crystallogr F Struct Biol Commun