CATH Classification

Domain Context

CATH Clusters

Superfamily 3.60.130.30
Functional Family

Enzyme Information

1.14.11.n2
Methylcytosine dioxygenase.
based on mapping to UniProt D2W6T1
DNA 5-methylcytosine + 2-oxoglutarate + O(2) = DNA 5-hydroxymethylcytosine + succinate + CO(2).
-!- Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC). -!- Might initiate a process leading to cytosine demethylation through deamination into 5-hydroxymethyluracil (5hmU) and subsequent replacement by unmethylated cytosine by the base excision repair system. -!- Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation.

UniProtKB Entries (1)

D2W6T1
TET1_NAEGR
Naegleria gruberi
Tet-like dioxygenase 1

PDB Structure

PDB 5CG9
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Structure of Naegleria Tet-like dioxygenase (NgTet1) in complexes with a reaction intermediate 5-hydroxymethylcytosine DNA.
Hashimoto, H., Pais, J.E., Dai, N., Correa, I.R., Zhang, X., Zheng, Y., Cheng, X.
Nucleic Acids Res.