CATH Classification

Domain Context

CATH Clusters

Superfamily 2.20.70.10
Functional Family E3 ubiquitin-protein ligase NEDD4-like protein

Enzyme Information

2.3.2.26
HECT-type E3 ubiquitin transferase.
based on mapping to UniProt P46934
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)- ubiquitinyl-[acceptor protein]-L-lysine.
-!- In the first step the enzyme transfers ubiquitin from the E2 ubiquitin-conjugating enzyme (EC 2.3.2.23) to a cysteine residue in its HECT domain (which is located in the C-terminal region), forming a thioester bond. -!- In a subsequent step the enzyme transfers the ubiquitin to an acceptor protein, resulting in the formation of an isopeptide bond between the C-terminal glycine residue of ubiquitin and the epsilon- amino group of an L-lysine residue of the acceptor protein. -!- Cf. EC 2.3.2.27 and EC 2.3.2.31.

UniProtKB Entries (1)

P46934
NEDD4_HUMAN
Homo sapiens
E3 ubiquitin-protein ligase NEDD4

PDB Structure

PDB 5AHT
External Links
Method SOLUTION NMR
Organism
Primary Citation
The Nedd4-1 Ww Domain Recognizes the Py Motif Peptide Through Coupled Folding and Binding Equilibria.
Panwalkar, V., Neudecker, P., Schmitz, M., Lecher, J., Schulte, M., Medini, K., Stoldt, M., Brimble, M.A., Willbold, D., Dingley, A.J.
Biochemistry