CATH Classification

Domain Context

CATH Clusters

Superfamily NADPH-cytochrome p450 Reductase; Chain A, domain 3
Functional Family NADPH--cytochrome P450 reductase

Enzyme Information

1.6.2.4
NADPH--hemoprotein reductase.
based on mapping to UniProt P00388
NADPH + n oxidized hemoprotein = NADP(+) + n reduced hemoprotein.
-!- This enzyme catalyzes the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P450 monooxygenases (e.g. EC 1.14.14.1) by stabilizing the one-electron reduced form of the flavin cofactors FAD and FMN. -!- It also reduces cytochrome b5 and cytochrome c. -!- The number n in the equation is 1 if the hemoprotein undergoes a 2-electron reduction, and is 2 if it undergoes a 1-electron reduction.

UniProtKB Entries (1)

P00388
NCPR_RAT
Rattus norvegicus
NADPH--cytochrome P450 reductase

PDB Structure

PDB 4YAF
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Mutants of Cytochrome P450 Reductase Lacking Either Gly-141 or Gly-143 Destabilize Its FMN Semiquinone.
Rwere, F., Xia, C., Im, S., Haque, M.M., Stuehr, D.J., Waskell, L., Kim, J.J.
J.Biol.Chem.