CATH Classification

Domain Context

CATH Clusters

Superfamily FAD/NAD(P)-binding domain
Functional Family

Enzyme Information

2.4.2.60
Cysteine-dependent adenosine diphosphate thiazole synthase.
based on mapping to UniProt P32318
NAD(+) + glycine + [ADP-thiazole synthase]-L-cysteine = nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + [ADP-thiazole synthase]- dehydroalanine + 3 H(2)O.
-!- This iron dependent enzyme, found in fungi, plants, and some archaea, is involved in the thiamine phosphate biosynthesis pathway. -!- It is a single turn-over enzyme since the cysteine residue is not regenerated in vivo. -!- The homologous enzyme in archaea (EC 2.4.2.59) uses sulfide as sulfur donor.

UniProtKB Entries (1)

P32318
THI4_YEAST
Saccharomyces cerevisiae S288C
Thiamine thiazole synthase

PDB Structure

PDB 4Y4L
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Structural Basis for Iron-Mediated Sulfur Transfer in Archael and Yeast Thiazole Synthases.
Zhang, X., Eser, B.E., Chanani, P.K., Begley, T.P., Ealick, S.E.
Biochemistry