CATH Classification

Domain Context

CATH Clusters

Superfamily NADH Oxidase
Functional Family Coenzyme F420:L-glutamate ligase

Enzyme Information

6.3.2.34
Coenzyme F420-1:gamma-L-glutamate ligase.
based on mapping to UniProt P9WP79
GTP + coenzyme F420-1 + L-glutamate = GDP + phosphate + coenzyme gamma- F420-2.
-!- This protein catalyzes the successive addition of two glutamate residues to cofactor F420 by two distinct and independent reactions. -!- In the first reaction (EC 6.3.2.31) the enzyme attaches a glutamate via its alpha-amine group to F420-0. -!- In the second reaction, which is described here, the enzyme catalyzes the addition of a second L-glutamate residue to the gamma-carboxyl of the first glutamate.
6.3.2.31
Coenzyme F420-0:L-glutamate ligase.
based on mapping to UniProt P9WP79
GTP + coenzyme F420-0 + L-glutamate = GDP + phosphate + coenzyme F420-1.
-!- This protein catalyzes the successive addition of two glutamate residues to cofactor F420 by two distinct and independent reactions. -!- In the reaction described here the enzyme attaches a glutamate via its alpha-amine group to F420-0. -!- In the second reaction (EC 6.3.2.34) it catalyzes the addition of a second L-glutamate residue to the gamma-carboxyl of the first glutamate.

UniProtKB Entries (1)

P9WP79
FBIB_MYCTU
Mycobacterium tuberculosis H37Rv
Coenzyme F420:L-glutamate ligase

PDB Structure

PDB 4XOM
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Elongation of the Poly-gamma-glutamate Tail of F420 Requires Both Domains of the F420: gamma-Glutamyl Ligase (FbiB) of Mycobacterium tuberculosis.
Bashiri, G., Rehan, A.M., Sreebhavan, S., Baker, H.M., Baker, E.N., Squire, C.J.
J.Biol.Chem.