CATH Classification

Domain Context

CATH Clusters

Superfamily Taq DNA Polymerase; Chain T, domain 4
Functional Family DNA polymerase I

Enzyme Information

2.7.7.7
DNA-directed DNA polymerase.
based on mapping to UniProt P19821
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
-!- Catalyzes DNA-template-directed extension of the 3'-end of a DNA strand by one nucleotide at a time. -!- Cannot initiate a chain de novo. -!- Requires a primer which may be DNA or RNA. -!- See also EC 2.7.7.49.

UniProtKB Entries (1)

P19821
DPO1_THEAQ
Thermus aquaticus
DNA polymerase I, thermostable

PDB Structure

PDB 4XIU
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
A Conservative Isoleucine to Leucine Mutation Causes Major Rearrangements and Cold Sensitivity in KlenTaq1 DNA Polymerase.
Wu, E.Y., Walsh, A.R., Materne, E.C., Hiltner, E.P., Zielinski, B., Miller, B.R., Mawby, L., Modeste, E., Parish, C.A., Barnes, W.M., Kermekchiev, M.B.
Biochemistry