CATH Classification

Domain Context

CATH Clusters

Superfamily NAD(P)-binding Rossmann-like Domain
Functional Family Ketol-acid reductoisomerase (NADP(+))

Enzyme Information

1.1.1.383
Ketol-acid reductoisomerase (NAD(P)(+)).
based on mapping to UniProt E0SRA9
(2R)-2,3-dihydroxy-3-methylbutanoate + NAD(P)(+) = (2S)-2-hydroxy-2- methyl-3-oxobutanoate + NAD(P)H.
-!- The enzyme, characterized from the bacteria Hydrogenobaculum sp. and Syntrophomonas wolfei subsp. wolfei and from the archaea Metallosphaera sedula and Ignisphaera aggregans, can use both NADH and NADPH with similar efficiency (cf. EC 1.1.1.86 and EC 1.1.1.382).

UniProtKB Entries (1)

E0SRA9
ILVC_IGNAA
Ignisphaera aggregans DSM 17230
Ketol-acid reductoisomerase (NAD(P)(+))

PDB Structure

PDB 4XDZ
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Cofactor specificity motifs and the induced fit mechanism in class I ketol-acid reductoisomerases.
Cahn, J.K., Brinkmann-Chen, S., Spatzal, T., Wiig, J.A., Buller, A.R., Einsle, O., Hu, Y., Ribbe, M.W., Arnold, F.H.
Biochem.J.