CATH Classification

Domain Context

CATH Clusters

Superfamily Nitrogenase molybdenum iron protein domain
Functional Family

Enzyme Information

1.18.6.1
Nitrogenase.
based on mapping to UniProt P00467
8 reduced ferredoxin + 8 H(+) + N(2) + 16 ATP + 16 H(2)O = 8 oxidized ferredoxin + H(2) + 2 NH(3) + 16 ADP + 16 phosphate.
-!- The enzyme is a complex of two components (namely dinitrogen reducatse and dinitrogenase). -!- Dinitrogen reductase is a [4Fe-4S] protein, which, in the presence of two molecules of ATP, transfers an electron from ferredoxin to the dinitrogenase component. -!- Dinitrogenase is a molybdenum-iron protein that reduces dinitrogen to two molecules of ammonia in three successive two-electron reductions via diazene and hydrazine. -!- The reduction is initiated by formation of hydrogen in stoichiometric amounts. -!- Acetylene is reduced to ethylene (but only very slowly to ethane), azide to nitrogen and ammonia, and cyanide to methane and ammonia. -!- In the absence of a suitable substrate, hydrogen is slowly formed. -!- Ferredoxin may be replaced by flavodoxin (see EC 1.19.6.1). -!- The enzyme does not reduce CO (cf. EC 1.18.6.2). -!- Formerly EC 1.18.2.1.

UniProtKB Entries (1)

P00467
NIFD_CLOPA
Clostridium pasteurianum
Nitrogenase molybdenum-iron protein alpha chain

PDB Structure

PDB 4WN9
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Substrate Pathways in the Nitrogenase MoFe Protein by Experimental Identification of Small Molecule Binding Sites.
Morrison, C.N., Hoy, J.A., Zhang, L., Einsle, O., Rees, D.C.
Biochemistry