CATH Classification

Domain Context

CATH Clusters

Superfamily 6.10.280.250
Functional Family

Enzyme Information

1.14.11.27
[Histone H3]-dimetyl-L-lysine-36 demethylase.
based on mapping to UniProt P59997
[Protein]-N(6),N(6)-dimethyl-L-lysine + 2 2-oxoglutarate + 2 O(2) = [protein]-L-lysine + 2 succinate + 2 formaldehyde + 2 CO(2).
-!- Of the seven potential methylation sites in histones H3 (K4, K9, K27, K36, K79) and H4 (K20, R3) from HeLa cells, the enzyme is specific for Lys-36. -!- Lysine residues exist in three methylation states (mono-, di- and trimethylated). -!- The enzyme preferentially demethylates the dimethyl form of Lys-36 (K36me2), which is its natural substrate, to form the monomethyl and unmethylated forms of Lys-36. -!- It can also demethylate the monomethyl- but not the trimethyl form of Lys-36.

UniProtKB Entries (2)

P59997
KDM2A_MOUSE
Mus musculus
Lysine-specific demethylase 2A
P68431
H31_HUMAN
Homo sapiens
Histone H3.1

PDB Structure

PDB 4TN7
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
A molecular threading mechanism underlies Jumonji lysine demethylase KDM2A regulation of methylated H3K36.
Cheng, Z., Cheung, P., Kuo, A.J., Yukl, E.T., Wilmot, C.M., Gozani, O., Patel, D.J.
Genes Dev.