CATH Classification

Domain Context

CATH Clusters

Superfamily Ubiquitin Conjugating Enzyme
Functional Family Ubiquitin-conjugating enzyme E2 2

Enzyme Information

2.3.2.23
E2 ubiquitin-conjugating enzyme.
based on mapping to UniProt P06104
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L- cysteine.
-!- The E2 ubiquitin-conjugating enzyme acquires the activated ubquitin from the E1 ubiquitin-activating enzyme (EC 6.2.1.45) and binds it via a transthioesterification reaction to itself. -!- In the human enzyme the catalytic center is located at Cys-87 where ubiquitin is bound via its C-terminal glycine in a thioester linkage. -!- Formerly EC 6.3.2.19.

UniProtKB Entries (2)

P62979
RS27A_HUMAN
Homo sapiens
Ubiquitin-40S ribosomal protein S27a
P06104
UBC2_YEAST
Saccharomyces cerevisiae S288C
Ubiquitin-conjugating enzyme E2 2

PDB Structure

PDB 4R62
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Role of a non-canonical surface of Rad6 in ubiquitin conjugating activity.
Kumar, P., Magala, P., Geiger-Schuller, K.R., Majumdar, A., Tolman, J.R., Wolberger, C.
Nucleic Acids Res.