CATH Classification

Domain Context

CATH Clusters

Superfamily 1.20.58.2210
Functional Family

Enzyme Information

1.14.11.27
[Histone H3]-lysine-36 demethylase.
based on mapping to UniProt P59997
(1) Protein N(6),N(6)-dimethyl-L-lysine + 2-oxoglutarate + O(2) = protein N(6)-methyl-L-lysine + succinate + formaldehyde + CO(2). (2) Protein N(6)-methyl-L-lysine + 2-oxoglutarate + O(2) = protein L-lysine + succinate + formaldehyde + CO(2).
-!- Of the seven potential methylation sites in histones H3 (K4, K9, K27, K36, K79) and H4 (K20, R3) from HeLa cells, the enzyme is specific for Lys-36. -!- Lysine residues exist in three methylation states (mono-, di- and trimethylated). -!- The enzyme preferentially demethylates the dimethyl form of Lys-36 (K36me2), which is its natural substrate, to form the monomethyl and unmethylated forms of Lys-36. -!- It can also demethylate the monomethyl- but not the trimethyl form of Lys-36.

UniProtKB Entries (1)

P59997
KDM2A_MOUSE
Mus musculus
Lysine-specific demethylase 2A

PDB Structure

PDB 4QXC
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
A molecular threading mechanism underlies Jumonji lysine demethylase KDM2A regulation of methylated H3K36.
Cheng, Z., Cheung, P., Kuo, A.J., Yukl, E.T., Wilmot, C.M., Gozani, O., Patel, D.J.
Genes Dev.