CATH Classification

Domain Context

CATH Clusters

Superfamily Helix hairpin bin
Functional Family Pyrrolysine--tRNA ligase

Enzyme Information

6.1.1.26
Pyrrolysine--tRNA(Pyl) ligase.
based on mapping to UniProt Q8PWY1
ATP + L-pyrrolysine + tRNA(Pyl) = AMP + diphosphate + L-pyrrolysyl- tRNA(Pyl).
-!- In organisms such as Methanosarcina barkeri that incorporate the modified amino acid pyrrolysine (Pyl) into certain methylamine methyltransferases, an unusual tRNA(Pyl), with a CUA anticodon, can be charged directly with pyrrolysine by this class II aminoacyl- -tRNA ligase. -!- Specific for pyrrolysine as substrate as it cannot be replaced by lysine or any of the other natural amino acids.

UniProtKB Entries (1)

Q8PWY1
PYLS_METMA
Methanosarcina mazei Go1
Pyrrolysine--tRNA ligase

PDB Structure

PDB 4Q6G
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Polyspecific pyrrolysyl-tRNA synthetases from directed evolution.
Guo, L.T., Wang, Y.S., Nakamura, A., Eiler, D., Kavran, J.M., Wong, M., Kiessling, L.L., Steitz, T.A., O'Donoghue, P., Soll, D.
Proc.Natl.Acad.Sci.USA