CATH Classification

Domain Context

CATH Clusters

Superfamily DNA-directed RNA polymerase, insert domain
Functional Family DNA-directed RNA polymerase subunit alpha

Enzyme Information

2.7.7.6
DNA-directed RNA polymerase.
based on mapping to UniProt Q5SHR6
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
-!- Catalyzes DNA-template-directed extension of the 3'-end of an RNA strand by one nucleotide at a time. -!- Can initiate a chain de novo. -!- In eukaryotes three forms of the enzyme have been distinguished on the basis of sensitivity of alpha-amanitin, and the type of RNA synthesized. -!- See also EC 2.7.7.19 and EC 2.7.7.48.

UniProtKB Entries (1)

Q5SKW1
Q5SKW1_THET8
Thermus thermophilus HB8
RNA polymerase sigma factor SigA

PDB Structure

PDB 4MQ9
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
GE23077 binds to the RNA polymerase 'i' and 'i+1' sites and prevents the binding of initiating nucleotides.
Zhang, Y., Degen, D., Ho, M.X., Sineva, E., Ebright, K.Y., Ebright, Y.W., Mekler, V., Vahedian-Movahed, H., Feng, Y., Yin, R., Tuske, S., Irschik, H., Jansen, R., Maffioli, S., Donadio, S., Arnold, E., Ebright, R.H.
Elife