CATH Classification

Domain Context

CATH Clusters

Superfamily Oligo-1,6-glucosidase; Domain 2
Functional Family Sucrose isomerase

Enzyme Information

3.2.1.10
Oligo-1,6-glucosidase.
based on mapping to UniProt O06994
Hydrolysis of (1->6)-alpha-D-glucosidic linkages in some oligosaccharides produced from starch and glycogen by alpha-amylase, and in isomaltose.
-!- This enzyme, like EC 3.2.1.33, can release an alpha-1->6-linked glucose, whereas the shortest chain that can be released by EC 3.2.1.41, EC 3.2.1.142 and EC 3.2.1.68 is maltose. -!- It also hydrolyzes isomaltulose (palatinose), isomaltotriose and panose, but has no action on glycogen or phosphorylase limit dextrin. -!- The enzyme from intestinal mucosa is a single polypeptide chain that also catalyzes the reaction of EC 3.2.1.48. -!- Differs from EC 3.2.1.33 in its preference for short-chain substrates and in its not requiring the 6-glucosylated residue to be at a branch point, i.e. linked at both C-1 and C-4.

UniProtKB Entries (1)

O06994
O16G1_BACSU
Bacillus subtilis subsp. subtilis str. 168
Oligo-1,6-glucosidase 1

PDB Structure

PDB 4M8U
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Change in heat capacity for enzyme catalysis determines temperature dependence of enzyme catalyzed rates.
Hobbs, J.K., Jiao, W., Easter, A.D., Parker, E.J., Schipper, L.A., Arcus, V.L.
Acs Chem.Biol.