CATH Classification

Domain Context

CATH Clusters

Superfamily Carbon-nitrogen hydrolase
Functional Family

Enzyme Information

3.5.1.4
Amidase.
based on mapping to UniProt Q9L543
A monocarboxylic acid amide + H(2)O = a monocarboxylate + NH(3).

UniProtKB Entries (1)

Q9L543
AMIE_BACSP
Bacillus sp. (in: Bacteria)
Aliphatic amidase

PDB Structure

PDB 4KZF
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
The mechanism of the amidases: mutating the glutamate adjacent to the catalytic triad inactivates the enzyme due to substrate mispositioning.
Weber, B.W., Kimani, S.W., Varsani, A., Cowan, D.A., Hunter, R., Venter, G.A., Gumbart, J.C., Sewell, B.T.
J.Biol.Chem.