CATH Classification

Domain Context

CATH Clusters

Superfamily Glucose Oxidase, domain 3
Functional Family

Enzyme Information

1.1.99.29
Pyranose dehydrogenase (acceptor).
based on mapping to UniProt Q3L245
(1) Pyranose + acceptor = pyranos-2-ulose + reduced acceptor. (2) Pyranose + acceptor = pyranos-3-ulose + reduced acceptor. (3) Pyranose + acceptor = pyranos-2,3-diulose + reduced acceptor. (4) A pyranoside + acceptor = a pyranosid-3-ulose + reduced acceptor. (5) A pyranoside + acceptor = a pyranosid-3,4-diulose + reduced acceptor.
-!- A number of aldoses and ketoses in pyranose form, as well as glycosides, gluco-oligosaccharides, sucrose and lactose can act as a donor. -!- 1,4-benzoquinone or ferricenium ion (ferrocene oxidized by removal of one electron) can serve as acceptor. -!- Unlike EC 1.1.3.10, this fungal enzyme does not interact with O(2) and exhibits extremely broad substrate tolerance with variable regioselectivity (C-3, C-2 or C-3 + C-2 or C-3 + C-4) for (di)oxidation of different sugars. -!- D-glucose is exclusively or preferentially oxidized at C-3 (depending on the enzyme source), but can also be oxidized at C-2 + C-3. -!- The enzyme also acts on 1->4-alpha- and 1->4-beta-gluco- oligosaccharides, non-reducing gluco-oligosaccharides and L-arabinose, which are not substrates of EC 1.1.3.10. -!- Sugars are oxidized in their pyranose but not in their furanose form.

UniProtKB Entries (1)

Q3L245
PDH1_LEUMG
Leucoagaricus meleagris
Pyranose dehydrogenase 1

PDB Structure

PDB 4H7U
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
The 1.6 A crystal structure of pyranose dehydrogenase from Agaricus meleagris rationalizes substrate specificity and reveals a flavin intermediate.
Tan, T.C., Spadiut, O., Wongnate, T., Sucharitakul, J., Krondorfer, I., Sygmund, C., Haltrich, D., Chaiyen, P., Peterbauer, C.K., Divne, C.
Plos One