CATH Classification

Domain Context

CATH Clusters

Superfamily Peptidase C65 Otubain, subdomain 1
Functional Family ubiquitin thioesterase OTUB2 isoform X2

Enzyme Information

3.4.19.12
Ubiquitinyl hydrolase 1.
based on mapping to UniProt Q96DC9
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
-!- Links to polypeptides smaller than 60 residues are hydrolyzed more readily than those to larger polypeptides. -!- Isoforms exist with quantitatively different specificities among the best known being UCH-L1 and UCH-L3, major proteins of the brain of mammals. -!- Inhibited by ubiquitin aldehyde (in which Gly76 is replaced by aminoacetaldehyde). -!- Belongs to peptidase family C12.

UniProtKB Entries (1)

Q96DC9
OTUB2_HUMAN
Homo sapiens
Ubiquitin thioesterase OTUB2

PDB Structure

PDB 4FJV
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
The human otubain2-ubiquitin structure provides insights into the cleavage specificity of poly-ubiquitin-linkages.
Altun, M., Walter, T.S., Kramer, H.B., Herr, P., Iphofer, A., Bostrom, J., David, Y., Komsany, A., Ternette, N., Navon, A., Stuart, D.I., Ren, J., Kessler, B.M.
Plos One