CATH Classification

Domain Context

CATH Clusters

Superfamily alpha/beta hydrolase
Functional Family Pimeloyl-[acyl-carrier protein] methyl ester esterase

Enzyme Information

3.1.1.85
Pimeloyl-[acyl-carrier protein] methyl ester esterase.
based on mapping to UniProt Q83PW0
Pimeloyl-[acyl-carrier protein] methyl ester + H(2)O = pimeloyl-[acyl- carrier protein] + methanol.
-!- Involved in biotin biosynthesis in Gram-negative bacteria. -!- The enzyme exhibits carboxylesterase activity, particularly toward substrates with short acyl chains. -!- Even though the enzyme can interact with coenzyme A thioesters, the in vivo role of the enzyme is to hydrolyze the methyl ester of pimeloyl-[acyl carrier protein], terminating the part of the biotin biosynthesis pathway that is catalyzed by the fatty acid elongation enzymes.

UniProtKB Entries (2)

Q83PW0
BIOH_SHIFL
Shigella flexneri
Pimeloyl-[acyl-carrier protein] methyl ester esterase
Q0T5U2
ACP_SHIF8
Shigella flexneri 5 str. 8401
Acyl carrier protein

PDB Structure

PDB 4ETW
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Structure of the enzyme-acyl carrier protein (ACP) substrate gatekeeper complex required for biotin synthesis.
Agarwal, V., Lin, S., Lukk, T., Nair, S.K., Cronan, J.E.
Proc.Natl.Acad.Sci.USA