CATH Classification

Domain Context

CATH Clusters

Superfamily 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, catalytic domain
Functional Family 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Enzyme Information

1.17.7.4
4-hydroxy-3-methylbut-2-enyl diphosphate reductase.
based on mapping to UniProt P62623
(1) Isopentenyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H(2)O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H(+). (2) Dimethylallyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H(2)O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H(+).
-!- Forms a system with a ferredoxin or a flavodoxin and an NAD(P)H-dependent reductase. -!- This is the last enzyme in the non-mevalonate pathway for isoprenoid biosynthesis. -!- This pathway, also known as the 1-deoxy-D-xylulose 5-phosphate (DOXP) or as the 2-C-methyl-D-erythritol-4-phosphate (MEP) pathway, is found in most bacteria and in plant chloroplasts. -!- The enzyme acts in the reverse direction, producing a 5:1 mixture of isopentenyl diphosphate and dimethylallyl diphosphate. -!- Formerly EC 1.17.1.2.

UniProtKB Entries (1)

P62623
ISPH_ECOLI
Escherichia coli K-12
4-hydroxy-3-methylbut-2-enyl diphosphate reductase

PDB Structure

PDB 4EB3
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Are free radicals involved in IspH catalysis? An EPR and crystallographic investigation.
Wang, W., Wang, K., Span, I., Jauch, J., Bacher, A., Groll, M., Oldfield, E.
J.Am.Chem.Soc.