CATH Classification

Domain Context

CATH Clusters

Superfamily N-terminal domain of ligase-like
Functional Family Long-chain-fatty-acid--CoA ligase FadD

Enzyme Information

1.13.12.7
Firefly luciferase.
based on mapping to UniProt P08659
D-firefly luciferin + O(2) + ATP = firefly oxyluciferin + CO(2) + AMP + diphosphate + light.
-!- The enzyme, which is found in fireflies (Lampyridae), is responsible for their biolouminescence. -!- The reaction begins with the formation of an acid anhydride between the carboxylic group of D-firefly luciferin and AMP, with the release of diphosphate. -!- An oxygenation follows, with release of the AMP group and formation of a very short-lived peroxide that cyclizes into a dioxetanone structure, which collapses, releasing a CO(2) molecule. -!- The spontaneous breakdown of the dioxetanone (rather than the hydrolysis of the adenylate) releases the energy (about 50 kcal/mole) that is necessary to generate the excited state of oxyluciferin. -!- The excited luciferin then emits a photon, returning to its ground state. -!- The enzyme has a secondary acyl-CoA ligase activity when acting on L-firefly luciferin.

UniProtKB Entries (1)

P08659
LUCI_PHOPY
Photinus pyralis
Luciferin 4-monooxygenase

PDB Structure

PDB 4E5D
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Firefly luciferase in chemical biology: a compendium of inhibitors, mechanistic evaluation of chemotypes, and suggested use as a reporter.
Thorne, N., Shen, M., Lea, W.A., Simeonov, A., Lovell, S., Auld, D.S., Inglese, J.
Chem.Biol.