CATH Classification

Domain Context

CATH Clusters

Superfamily Alkaline Phosphatase, subunit A
Functional Family Arylsulfatase

Enzyme Information
Arylsulfatase (type I).
based on mapping to UniProt P51691
A phenol sulfate + H(2)O = a phenol + sulfate.
-!- Sulfatase enzymes are classified as type I, in which the key catalytic residue is 3-oxo-L-alanine, type II, which are non-heme iron-dependent dioxygenases, or type III, whose catalytic domain adopts a metallo-beta-lactamase fold and binds two zinc ions as cofactors. -!- Arylsulfatases are type I enzymes, found in both prokaryotes and eukaryotes, with rather similar specificities. -!- The key catalytic residue 3-oxo-L-alanine initiates the reaction through a nucleophilic attack on the sulfur atom in the substrate. -!- This residue is generated by posttranslational modification of a conserved cysteine or serine residue by EC, EC 1.1.98.-, serine-type anaerobic sulfatase-maturating enzyme, or EC 1.8.98.-, cysteine-type anaerobic sulfatase-maturating enzyme.

UniProtKB Entries (1)

Pseudomonas aeruginosa PAO1

PDB Structure

External Links
Primary Citation
Evolutionary repurposing of a sulfatase: A new Michaelis complex leads to efficient transition state charge offset.
Miton, C.M., Jonas, S., Fischer, G., Duarte, F., Mohamed, M.F., van Loo, B., Kintses, B., Kamerlin, S.C.L., Tokuriki, N., Hyvonen, M., Hollfelder, F.
Proc. Natl. Acad. Sci. U.S.A.