CATH Classification

Domain Context

CATH Clusters

Superfamily 3.40.630.170
Functional Family Glycylpeptide N-tetradecanoyltransferase

Enzyme Information

2.3.1.97
Glycylpeptide N-tetradecanoyltransferase.
based on mapping to UniProt O60551
Tetradecanoyl-CoA + an N-terminal-glycyl-[protein] = CoA + an N-terminal- N-tetradecanoylglycyl-[protein].
-!- The enzyme catalyzes the transfer of myristic acid from myristoyl-CoA to the amino group of the N-terminal glycine residue in a variety of eukaryotic proteins. -!- It uses an ordered Bi Bi reaction in which myristoyl-CoA binds to the enzyme prior to the binding of the peptide substrate, and CoA release precedes the release of the myristoylated peptide. -!- The enzyme from yeast is profoundly affected by amino acids further from the N-terminus, and is particularly stimulated by a serine residue at position 5.

UniProtKB Entries (1)

O60551
NMT2_HUMAN
Homo sapiens
Glycylpeptide N-tetradecanoyltransferase 2

PDB Structure

PDB 4C2X
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Global Profiling of Co- and Post-Translationally N-Myristoylated Proteomes in Human Cells.
Thinon, E., Serwa, R.A., Broncel, M., Brannigan, J.A., Brassat, U., Wright, M.H., Heal, W.P., Wilkinson, A.J., Mann, D.J., Tate, E.W.
Nat.Commun.