CATH Classification

Domain Context

CATH Clusters

Superfamily 3.90.640.50
Functional Family

Enzyme Information

3.1.21.3
Type I site-specific deoxyribonuclease.
based on mapping to UniProt P10486
Endonucleolytic cleavage of DNA to give random double-stranded fragments with terminal 5'-phosphates, ATP is simultaneously hydrolyzed.
-!- Large group of enzymes that have an absolute requirement for ATP (or dATP) and S-adenosyl-L-methionine. -!- They recognize specific short DNA sequences and cleave at sites remote from the recognition sequence. -!- Multifunctional proteins which also catalyze the reactions of EC 2.1.1.72 and EC 2.1.1.73, with similar site specificity. -!- Formerly EC 3.1.24.1 and EC 3.1.24.2. -!- See the REBASE database for a complete list of these enzymes: http://rebase.neb.com/rebase/

UniProtKB Entries (1)

P10486
T1R1_ECOLX
Escherichia coli
Type I restriction enzyme EcoR124II R protein

PDB Structure

PDB 4BE7
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Functional Coupling of Duplex Translocation to DNA Cleavage in a Type I Restriction Enzyme.
Csefalvay, E., Lapkouski, M., Guzanova, A., Csefalvay, L., Baikova, T., Shevelev, I., Bialevich, V., Shamayeva, K., Janscak, P., Kuta Smatanova, I., Panjikar, S., Carey, J., Weiserova, M., Ettrich, R.
Plos One